Effect of 5-Aminolevulinic Acid (ALA) on Leaf Diurnal Photosynthetic Characteristics and Antioxidant Activity in Pear (Pyrus Pyrifolia Nakai)
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Fine mapping of the gene for susceptibility to black spot disease in Japanese pear (Pyrus pyrifolia Nakai)
Black spot disease, which is caused by the Japanese pear pathotype of the filamentous fungus Alternaria alternata (Fries) Keissler, is one of the most harmful diseases in Japanese pear cultivation. We mapped a gene for susceptibility to black spot disease in the Japanese pear (Pyrus pyrifolia Nakai) cultivar 'Kinchaku' (Aki gene) at the top of linkage group 11, similar to the positions of the s...
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The present study was conducted to evaluate the ameliorating effect of 5-aminolevulinic acid (ALA) on physiological and biochemical changes of induced NaCl-salinity stress on in vitro shoot and callus cultured of red-fleshed apple. Shoot and callus segments of the red-flesh apple of Local Iranian genotype were cultured on MS medium containing different levels of NaCl (0, 30, 60 and 90 mM) and A...
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We evaluated antioxidant activities of heated pear juice (HPJ) exposed to 120, 130, and 140°C for 2 hr. HPJ was partitioned using n-hexane, chloroform, ethyl acetate, n-butanol, and water. The ethyl acetate fraction treated at 130°C for 2 hr showed strong antioxidant activity; thus, this extract was isolated and purified using silica gel column chromatography and preparative high performance li...
متن کاملPhysical mapping of black spot disease resistance/susceptibility-related genome regions in Japanese pear (Pyrus pyrifolia) by BAC-FISH
Black spot disease, caused by Alternaria alternata Japanese pear pathotype, is one of the most harmful diseases in Japanese pear cultivation. In the present study, the locations of black spot disease resistance/susceptibility-related genome regions were studied by fluorescence in situ hybridization using BAC clone (BAC-FISH) on Japanese pear (Pyrus pyrifolia (Burm. f.) Nakai) chromosomes. Root ...
متن کاملAgrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia)
Genetic transformation was successfully established producing both transformed adventitious shoots and calli in Japanese pear (Pyrus pyrifolia Nakai) by using cotyledons as explants. Cotyledons of five cultivars were co-cultivated with Agrobacterium tumefaciens strain LBA4404 carrying the pBIN19-sgfp, which contained a green fluorescent protein gene and the neomycin phosphotransferase gene. In ...
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